Priming with rAAV encoding RBD of SARS-CoV S protein and boosting with RBD-specific peptides for T cell epitopes elevated humoral and cellular immune responses against SARS-CoV infection
Identifieur interne : 003479 ( Main/Exploration ); précédent : 003478; suivant : 003480Priming with rAAV encoding RBD of SARS-CoV S protein and boosting with RBD-specific peptides for T cell epitopes elevated humoral and cellular immune responses against SARS-CoV infection
Auteurs : LANYING DU [Hong Kong, États-Unis] ; GUANGYU ZHAO [République populaire de Chine] ; YONGPING LIN [Hong Kong] ; CHRIS CHAN [Hong Kong] ; YUXIAN HE [États-Unis] ; SHIBO JIANG [États-Unis] ; CHANGYOU WU [République populaire de Chine] ; Dong-Yan Jin [Hong Kong] ; Kwok-Yung Yuen [Hong Kong] ; YUSEN ZHOU [République populaire de Chine] ; Bo-Jian Zheng [Hong Kong]Source :
- Vaccine [ 0264-410X ] ; 2008.
Descripteurs français
- Pascal (Inist)
- Wicri :
- topic : Vaccination.
English descriptors
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Abstract
Development of vaccines against severe acute respiratory syndrome (SARS) coronavirus (SARS-CoV) is crucial in the prevention of SARS reemergence. The receptor-binding domain (RBD) of SARS-CoV spike (S) protein is an important target in developing safe and effective SARS vaccines. Our previous study has demonstrated that vaccination with adenoassociated virus encoding RBD (RBD-rAAV) induces high titer of neutralizing antibodies. In this study, we further assessed the immune responses and protective effect of the immunization with RBD-rAAV prime/RBD-specific T cell peptide boost. Compared with the RBD-rAAV prime/boost vaccination, RBD-rAAV prime/RBD-peptide (RBD-Pep) boost induced similar levels of Th1 and neutralizing antibody responses that protected the vaccinated mice from subsequent SARS-CoV challenge, but stronger Th2 and CTL responses. No significant immune responses and protective effects were detected in mice vaccinated with RBD-Pep or blank AAV alone. Since T cell epitopes are highly conserved and boosting with peptides may induce the production of effector memory T cells, which may be effective against viruses with mutations in the neutralizing epitopes, our results suggest that the vaccination protocol used may be ideal for providing effective, broad and long-term protection against SARS-CoV infection.
Affiliations:
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<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en" level="a">Priming with rAAV encoding RBD of SARS-CoV S protein and boosting with RBD-specific peptides for T cell epitopes elevated humoral and cellular immune responses against SARS-CoV infection</title><author><name sortKey="Lanying Du" sort="Lanying Du" uniqKey="Lanying Du" last="Lanying Du">LANYING DU</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Department of Microbiology, The University of Hong Kong</s1><s2>Pokfulam</s2><s3>HKG</s3><sZ>1 aut.</sZ><sZ>3 aut.</sZ><sZ>4 aut.</sZ><sZ>9 aut.</sZ><sZ>11 aut.</sZ></inist:fA14><country>Hong Kong</country><wicri:noRegion>Pokfulam</wicri:noRegion></affiliation><affiliation wicri:level="2"><inist:fA14 i1="02"><s1>Lindsley F. Kimball Research Institute, The New York Blood Center</s1><s2>New York, NY 10021</s2><s3>USA</s3><sZ>1 aut.</sZ><sZ>5 aut.</sZ><sZ>6 aut.</sZ></inist:fA14><country>États-Unis</country><placeName><region type="state">État de New York</region></placeName></affiliation></author><author><name sortKey="Guangyu Zhao" sort="Guangyu Zhao" uniqKey="Guangyu Zhao" last="Guangyu Zhao">GUANGYU ZHAO</name><affiliation wicri:level="1"><inist:fA14 i1="03"><s1>State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology</s1><s2>Beijing 100071</s2><s3>CHN</s3><sZ>2 aut.</sZ><sZ>10 aut.</sZ></inist:fA14><country>République populaire de Chine</country><placeName><settlement type="city">Pékin</settlement></placeName></affiliation></author><author><name sortKey="Yongping Lin" sort="Yongping Lin" uniqKey="Yongping Lin" last="Yongping Lin">YONGPING LIN</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Department of Microbiology, The University of Hong Kong</s1><s2>Pokfulam</s2><s3>HKG</s3><sZ>1 aut.</sZ><sZ>3 aut.</sZ><sZ>4 aut.</sZ><sZ>9 aut.</sZ><sZ>11 aut.</sZ></inist:fA14><country>Hong Kong</country><wicri:noRegion>Pokfulam</wicri:noRegion></affiliation></author><author><name sortKey="Chris Chan" sort="Chris Chan" uniqKey="Chris Chan" last="Chris Chan">CHRIS CHAN</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Department of Microbiology, The University of Hong Kong</s1><s2>Pokfulam</s2><s3>HKG</s3><sZ>1 aut.</sZ><sZ>3 aut.</sZ><sZ>4 aut.</sZ><sZ>9 aut.</sZ><sZ>11 aut.</sZ></inist:fA14><country>Hong Kong</country><wicri:noRegion>Pokfulam</wicri:noRegion></affiliation></author><author><name sortKey="Yuxian He" sort="Yuxian He" uniqKey="Yuxian He" last="Yuxian He">YUXIAN HE</name><affiliation wicri:level="2"><inist:fA14 i1="02"><s1>Lindsley F. Kimball Research Institute, The New York Blood Center</s1><s2>New York, NY 10021</s2><s3>USA</s3><sZ>1 aut.</sZ><sZ>5 aut.</sZ><sZ>6 aut.</sZ></inist:fA14><country>États-Unis</country><placeName><region type="state">État de New York</region></placeName></affiliation></author><author><name sortKey="Shibo Jiang" sort="Shibo Jiang" uniqKey="Shibo Jiang" last="Shibo Jiang">SHIBO JIANG</name><affiliation wicri:level="2"><inist:fA14 i1="02"><s1>Lindsley F. Kimball Research Institute, The New York Blood Center</s1><s2>New York, NY 10021</s2><s3>USA</s3><sZ>1 aut.</sZ><sZ>5 aut.</sZ><sZ>6 aut.</sZ></inist:fA14><country>États-Unis</country><placeName><region type="state">État de New York</region></placeName></affiliation></author><author><name sortKey="Changyou Wu" sort="Changyou Wu" uniqKey="Changyou Wu" last="Changyou Wu">CHANGYOU WU</name><affiliation wicri:level="3"><inist:fA14 i1="04"><s1>Department of Immunology, Zhongshan School of Medicine, Sun Yat-sen University</s1><s2>Guangzhou</s2><s3>CHN</s3><sZ>7 aut.</sZ></inist:fA14><country>République populaire de Chine</country><placeName><settlement type="city">Jiangmen</settlement><region type="province">Guangdong</region></placeName></affiliation></author><author><name sortKey="Jin, Dong Yan" sort="Jin, Dong Yan" uniqKey="Jin D" first="Dong-Yan" last="Jin">Dong-Yan Jin</name><affiliation wicri:level="1"><inist:fA14 i1="05"><s1>Department of Biochemistry, The University of Hong Kong</s1><s2>Pokfulam</s2><s3>HKG</s3><sZ>8 aut.</sZ></inist:fA14><country>Hong Kong</country><wicri:noRegion>Pokfulam</wicri:noRegion></affiliation></author><author><name sortKey="Yuen, Kwok Yung" sort="Yuen, Kwok Yung" uniqKey="Yuen K" first="Kwok-Yung" last="Yuen">Kwok-Yung Yuen</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Department of Microbiology, The University of Hong Kong</s1><s2>Pokfulam</s2><s3>HKG</s3><sZ>1 aut.</sZ><sZ>3 aut.</sZ><sZ>4 aut.</sZ><sZ>9 aut.</sZ><sZ>11 aut.</sZ></inist:fA14><country>Hong Kong</country><wicri:noRegion>Pokfulam</wicri:noRegion></affiliation></author><author><name sortKey="Yusen Zhou" sort="Yusen Zhou" uniqKey="Yusen Zhou" last="Yusen Zhou">YUSEN ZHOU</name><affiliation wicri:level="1"><inist:fA14 i1="03"><s1>State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology</s1><s2>Beijing 100071</s2><s3>CHN</s3><sZ>2 aut.</sZ><sZ>10 aut.</sZ></inist:fA14><country>République populaire de Chine</country><placeName><settlement type="city">Pékin</settlement></placeName></affiliation></author><author><name sortKey="Zheng, Bo Jian" sort="Zheng, Bo Jian" uniqKey="Zheng B" first="Bo-Jian" last="Zheng">Bo-Jian Zheng</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Department of Microbiology, The University of Hong Kong</s1><s2>Pokfulam</s2><s3>HKG</s3><sZ>1 aut.</sZ><sZ>3 aut.</sZ><sZ>4 aut.</sZ><sZ>9 aut.</sZ><sZ>11 aut.</sZ></inist:fA14><country>Hong Kong</country><wicri:noRegion>Pokfulam</wicri:noRegion></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">INIST</idno><idno type="inist">08-0427329</idno><date when="2008">2008</date><idno type="stanalyst">PASCAL 08-0427329 INIST</idno><idno type="RBID">Pascal:08-0427329</idno><idno type="wicri:Area/PascalFrancis/Corpus">000255</idno><idno type="wicri:Area/PascalFrancis/Curation">000733</idno><idno type="wicri:Area/PascalFrancis/Checkpoint">000259</idno><idno type="wicri:explorRef" wicri:stream="PascalFrancis" wicri:step="Checkpoint">000259</idno><idno type="wicri:doubleKey">0264-410X:2008:Lanying Du:priming:with:raav</idno><idno type="wicri:Area/Main/Merge">003585</idno><idno type="wicri:Area/Main/Curation">003479</idno><idno type="wicri:Area/Main/Exploration">003479</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en" level="a">Priming with rAAV encoding RBD of SARS-CoV S protein and boosting with RBD-specific peptides for T cell epitopes elevated humoral and cellular immune responses against SARS-CoV infection</title><author><name sortKey="Lanying Du" sort="Lanying Du" uniqKey="Lanying Du" last="Lanying Du">LANYING DU</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Department of Microbiology, The University of Hong Kong</s1><s2>Pokfulam</s2><s3>HKG</s3><sZ>1 aut.</sZ><sZ>3 aut.</sZ><sZ>4 aut.</sZ><sZ>9 aut.</sZ><sZ>11 aut.</sZ></inist:fA14><country>Hong Kong</country><wicri:noRegion>Pokfulam</wicri:noRegion></affiliation><affiliation wicri:level="2"><inist:fA14 i1="02"><s1>Lindsley F. Kimball Research Institute, The New York Blood Center</s1><s2>New York, NY 10021</s2><s3>USA</s3><sZ>1 aut.</sZ><sZ>5 aut.</sZ><sZ>6 aut.</sZ></inist:fA14><country>États-Unis</country><placeName><region type="state">État de New York</region></placeName></affiliation></author><author><name sortKey="Guangyu Zhao" sort="Guangyu Zhao" uniqKey="Guangyu Zhao" last="Guangyu Zhao">GUANGYU ZHAO</name><affiliation wicri:level="1"><inist:fA14 i1="03"><s1>State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology</s1><s2>Beijing 100071</s2><s3>CHN</s3><sZ>2 aut.</sZ><sZ>10 aut.</sZ></inist:fA14><country>République populaire de Chine</country><placeName><settlement type="city">Pékin</settlement></placeName></affiliation></author><author><name sortKey="Yongping Lin" sort="Yongping Lin" uniqKey="Yongping Lin" last="Yongping Lin">YONGPING LIN</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Department of Microbiology, The University of Hong Kong</s1><s2>Pokfulam</s2><s3>HKG</s3><sZ>1 aut.</sZ><sZ>3 aut.</sZ><sZ>4 aut.</sZ><sZ>9 aut.</sZ><sZ>11 aut.</sZ></inist:fA14><country>Hong Kong</country><wicri:noRegion>Pokfulam</wicri:noRegion></affiliation></author><author><name sortKey="Chris Chan" sort="Chris Chan" uniqKey="Chris Chan" last="Chris Chan">CHRIS CHAN</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Department of Microbiology, The University of Hong Kong</s1><s2>Pokfulam</s2><s3>HKG</s3><sZ>1 aut.</sZ><sZ>3 aut.</sZ><sZ>4 aut.</sZ><sZ>9 aut.</sZ><sZ>11 aut.</sZ></inist:fA14><country>Hong Kong</country><wicri:noRegion>Pokfulam</wicri:noRegion></affiliation></author><author><name sortKey="Yuxian He" sort="Yuxian He" uniqKey="Yuxian He" last="Yuxian He">YUXIAN HE</name><affiliation wicri:level="2"><inist:fA14 i1="02"><s1>Lindsley F. Kimball Research Institute, The New York Blood Center</s1><s2>New York, NY 10021</s2><s3>USA</s3><sZ>1 aut.</sZ><sZ>5 aut.</sZ><sZ>6 aut.</sZ></inist:fA14><country>États-Unis</country><placeName><region type="state">État de New York</region></placeName></affiliation></author><author><name sortKey="Shibo Jiang" sort="Shibo Jiang" uniqKey="Shibo Jiang" last="Shibo Jiang">SHIBO JIANG</name><affiliation wicri:level="2"><inist:fA14 i1="02"><s1>Lindsley F. Kimball Research Institute, The New York Blood Center</s1><s2>New York, NY 10021</s2><s3>USA</s3><sZ>1 aut.</sZ><sZ>5 aut.</sZ><sZ>6 aut.</sZ></inist:fA14><country>États-Unis</country><placeName><region type="state">État de New York</region></placeName></affiliation></author><author><name sortKey="Changyou Wu" sort="Changyou Wu" uniqKey="Changyou Wu" last="Changyou Wu">CHANGYOU WU</name><affiliation wicri:level="3"><inist:fA14 i1="04"><s1>Department of Immunology, Zhongshan School of Medicine, Sun Yat-sen University</s1><s2>Guangzhou</s2><s3>CHN</s3><sZ>7 aut.</sZ></inist:fA14><country>République populaire de Chine</country><placeName><settlement type="city">Jiangmen</settlement><region type="province">Guangdong</region></placeName></affiliation></author><author><name sortKey="Jin, Dong Yan" sort="Jin, Dong Yan" uniqKey="Jin D" first="Dong-Yan" last="Jin">Dong-Yan Jin</name><affiliation wicri:level="1"><inist:fA14 i1="05"><s1>Department of Biochemistry, The University of Hong Kong</s1><s2>Pokfulam</s2><s3>HKG</s3><sZ>8 aut.</sZ></inist:fA14><country>Hong Kong</country><wicri:noRegion>Pokfulam</wicri:noRegion></affiliation></author><author><name sortKey="Yuen, Kwok Yung" sort="Yuen, Kwok Yung" uniqKey="Yuen K" first="Kwok-Yung" last="Yuen">Kwok-Yung Yuen</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Department of Microbiology, The University of Hong Kong</s1><s2>Pokfulam</s2><s3>HKG</s3><sZ>1 aut.</sZ><sZ>3 aut.</sZ><sZ>4 aut.</sZ><sZ>9 aut.</sZ><sZ>11 aut.</sZ></inist:fA14><country>Hong Kong</country><wicri:noRegion>Pokfulam</wicri:noRegion></affiliation></author><author><name sortKey="Yusen Zhou" sort="Yusen Zhou" uniqKey="Yusen Zhou" last="Yusen Zhou">YUSEN ZHOU</name><affiliation wicri:level="1"><inist:fA14 i1="03"><s1>State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology</s1><s2>Beijing 100071</s2><s3>CHN</s3><sZ>2 aut.</sZ><sZ>10 aut.</sZ></inist:fA14><country>République populaire de Chine</country><placeName><settlement type="city">Pékin</settlement></placeName></affiliation></author><author><name sortKey="Zheng, Bo Jian" sort="Zheng, Bo Jian" uniqKey="Zheng B" first="Bo-Jian" last="Zheng">Bo-Jian Zheng</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Department of Microbiology, The University of Hong Kong</s1><s2>Pokfulam</s2><s3>HKG</s3><sZ>1 aut.</sZ><sZ>3 aut.</sZ><sZ>4 aut.</sZ><sZ>9 aut.</sZ><sZ>11 aut.</sZ></inist:fA14><country>Hong Kong</country><wicri:noRegion>Pokfulam</wicri:noRegion></affiliation></author></analytic><series><title level="j" type="main">Vaccine</title><title level="j" type="abbreviated">Vaccine</title><idno type="ISSN">0264-410X</idno><imprint><date when="2008">2008</date></imprint></series></biblStruct></sourceDesc><seriesStmt><title level="j" type="main">Vaccine</title><title level="j" type="abbreviated">Vaccine</title><idno type="ISSN">0264-410X</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Antigenic determinant</term><term>Cellular immunity</term><term>Dependovirus</term><term>Humoral immunity</term><term>Immune response</term><term>Mouse</term><term>Peptides</term><term>Protein</term><term>Recombinant virus</term><term>Severe acute respiratory syndrome virus</term><term>T-Lymphocyte</term><term>Vaccination</term></keywords><keywords scheme="Pascal" xml:lang="fr"><term>Virus syndrome respiratoire aigu sévère</term><term>Dependovirus</term><term>Souris</term><term>Protéine</term><term>Peptide</term><term>Lymphocyte T</term><term>Déterminant antigénique</term><term>Immunité humorale</term><term>Immunité cellulaire</term><term>Réponse immune</term><term>Virus recombinant</term><term>Vaccination</term></keywords><keywords scheme="Wicri" type="topic" xml:lang="fr"><term>Vaccination</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Development of vaccines against severe acute respiratory syndrome (SARS) coronavirus (SARS-CoV) is crucial in the prevention of SARS reemergence. The receptor-binding domain (RBD) of SARS-CoV spike (S) protein is an important target in developing safe and effective SARS vaccines. Our previous study has demonstrated that vaccination with adenoassociated virus encoding RBD (RBD-rAAV) induces high titer of neutralizing antibodies. In this study, we further assessed the immune responses and protective effect of the immunization with RBD-rAAV prime/RBD-specific T cell peptide boost. Compared with the RBD-rAAV prime/boost vaccination, RBD-rAAV prime/RBD-peptide (RBD-Pep) boost induced similar levels of Th1 and neutralizing antibody responses that protected the vaccinated mice from subsequent SARS-CoV challenge, but stronger Th2 and CTL responses. No significant immune responses and protective effects were detected in mice vaccinated with RBD-Pep or blank AAV alone. Since T cell epitopes are highly conserved and boosting with peptides may induce the production of effector memory T cells, which may be effective against viruses with mutations in the neutralizing epitopes, our results suggest that the vaccination protocol used may be ideal for providing effective, broad and long-term protection against SARS-CoV infection.</div></front></TEI><affiliations><list><country><li>Hong Kong</li><li>République populaire de Chine</li><li>États-Unis</li></country><region><li>Guangdong</li><li>État de New York</li></region><settlement><li>Jiangmen</li><li>Pékin</li></settlement></list><tree><country name="Hong Kong"><noRegion><name sortKey="Lanying Du" sort="Lanying Du" uniqKey="Lanying Du" last="Lanying Du">LANYING DU</name></noRegion><name sortKey="Chris Chan" sort="Chris Chan" uniqKey="Chris Chan" last="Chris Chan">CHRIS CHAN</name><name sortKey="Jin, Dong Yan" sort="Jin, Dong Yan" uniqKey="Jin D" first="Dong-Yan" last="Jin">Dong-Yan Jin</name><name sortKey="Yongping Lin" sort="Yongping Lin" uniqKey="Yongping Lin" last="Yongping Lin">YONGPING LIN</name><name sortKey="Yuen, Kwok Yung" sort="Yuen, Kwok Yung" uniqKey="Yuen K" first="Kwok-Yung" last="Yuen">Kwok-Yung Yuen</name><name sortKey="Zheng, Bo Jian" sort="Zheng, Bo Jian" uniqKey="Zheng B" first="Bo-Jian" last="Zheng">Bo-Jian Zheng</name></country><country name="États-Unis"><region name="État de New York"><name sortKey="Lanying Du" sort="Lanying Du" uniqKey="Lanying Du" last="Lanying Du">LANYING DU</name></region><name sortKey="Shibo Jiang" sort="Shibo Jiang" uniqKey="Shibo Jiang" last="Shibo Jiang">SHIBO JIANG</name><name sortKey="Yuxian He" sort="Yuxian He" uniqKey="Yuxian He" last="Yuxian He">YUXIAN HE</name></country><country name="République populaire de Chine"><noRegion><name sortKey="Guangyu Zhao" sort="Guangyu Zhao" uniqKey="Guangyu Zhao" last="Guangyu Zhao">GUANGYU ZHAO</name></noRegion><name sortKey="Changyou Wu" sort="Changyou Wu" uniqKey="Changyou Wu" last="Changyou Wu">CHANGYOU WU</name><name sortKey="Yusen Zhou" sort="Yusen Zhou" uniqKey="Yusen Zhou" last="Yusen Zhou">YUSEN ZHOU</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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